Detection of trunk pathogen inoculum in young vineyards to encourage adoption of preventative practices.

Lawrence.screenshotDaniel Lawrence

Objectives are:
-Possible pathogen detection tools for growers.
-Evaluate and improve culture and molecular detection methods.
-Promote preventative practices to minimize pruning wound infections.
-Develop prediction models to improve decision making processes for pruning times and possible treatments.

Link to the presentaiton: “Detection of trunk pathogen inoculum in young vineyards to encourage adoption of preventative practices

Accurate identification of trunk fungi

Goal 2 – Accurate identification of trunk fungi

Activities – Allow for proper disease management and control practices

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Results & Outputs – Developed a comprehensive DNA sequence database of trunk inhabiting fungi

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Significant Outcomes & Impacts – Accurate species identification will lead to proper disease management practices.

Infection in Almonds

Goals– To identify rates of wood-canker infections within almond orchards, determine management practices, identify the pathogens present in the orchard, and climate conditions conducive for sporulation in order to increase orchard 

Activities
Identification and surveying of infested orchards, isolation and identification of wood canker fungi, and spore trapping.

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Results & Outputs
Identified pruning wounds, growth cracks, and wood breakage serve as primary points of infection. Found that spore trapping of wood canker fungi is in association with rain events. Successfully extended these initial findings to almond growing clientele in CA.

Significant Outcomes & Impacts
Development of a preliminary model and management practices from field observations and spore trappings.

Develop a detection tool for spores of wood-canker pathogens

Goal 1 – Develop a detection tool for spores of wood-canker pathogens (aka trunk pathogens), combined with new molecular markers for rapid identification.

Activities – Evaluated spore traps in young, apparently-healthy vineyards vs. mature, diseased vineyards.

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Results & Outputs – Demonstrated that both young and mature vineyards are at similar risk of infection by spores of the same trunk pathogens.

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Significant Outcomes & Impacts – Growers routinely forego preventative practices in young vineyards because trunk diseases are not a serious problem until year 8.  Our results make it clear – disease prevention should be adopted in young vineyards.